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Nanoparticles

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Gold nanoprisms could be appropriate candidates for in vitro cellular thermoablation due to their efficient internalization and heating capacity

 CharacterizationServices

 

 

The physical and chemical properties of magnetic nanoparticles largely depend on the synthesis method and chemical structure. The 25nm magnetic nanoparticles are highly monodisperse nano-octahedrons of magnetite capped with citrate anions. These nanoparticles can be employed as platforms for many applications such as magnetic separation, hyperthermia, biosensors.

CITRATE STABILIZED:

Attachment of (bio)molecules containing thiols groups –SH.

CARBOXILATED :

Can be activated by EDC and NHS to form an intermediate that can subsequently react with primary amine groups on a specific protein or any other ligand to be conjugated.

OCTAHEDRAL

APPLICATIONS:

> Magnetic separation
> Biosensing
> Magnetic hyperthermia
> Drug delivery

If your company needs to increase its medical device portfolio or have discover a specific biomarker that needs to be detected in very low quantities, we will be happy to introduce you our device.
heatsens_codevelopment

For more information:

> Visit the project specific website: heatsens.com

> Contact: sales@nanoimmunotech.es

Along these years, we have developed a bioconjugation knowhow to provide our customers the best solution for their projects, meaning that nowadays we also give you the possibility to design multifunctional structures for more accurate outcomes.

  Heatsens_techology

 

These biosensors can be applied to a broad range of sectors and applications, from the development of point-of-care devices for their use in a hospital o directly at the patient home in the clinical diagnosis field, to its use in a chicken farm for the detection of pathogenic microorganisms, or the detection of contaminants in water, and much more.

 Heatsens_sectors

Nanoimmunotech offers different potential formats of HEATSENS® technology for diverse customer needs.

 

Biodistribution, acute toxicity and maximum tolerated dose (OECD 423). Necropsy report of animals and collection of organs showing abnormalities.

Measurement of the immune response IgG and IgM to a foreign antigen in rats treated daily with or without a sample. Histological report of organs, blood chemistry and hemogram.

Determination of contact properties of the nanomaterials with blood cells or plasma proteins.

Coagulation: intrinsic, extrinsic and common coagulation pathways are assessed by partial thromboplastin time (APTT) prothrombin time (PT) and Thrombin time (TT), respectively. Based on free interference Viscosity Detection System (VDS).

Haemolysis: colorimetric measurement of haemoglobin released.

Platelet activation: activated platelets detected by flow cytometry and aggregation by microscopy.

Immunomodulation in PBMCs (peripheral blood mononuclear cells), macrophages and cell lines.

Phagocytosis: Functional evaluation and microscopy monitoring of nanoparticle internalization by phagocytic cells.

Inflammasome: Caspase-1 activation by flow cytometry and induction of IL-1β by enzyme immunoassay (ELISA).

Leukocyte proliferation: Clonal proliferation of PBMCs measured by flow cytometry.

Immunological response: Inflammatory cytokine profile and Th1, Th2, Th17 response of PBMCs measured by flow cytometry or Luminex multiplex technology.

Complement activation: Identification in serum of C3/C3b by western-blot. Quantification of C3b by enzyme immunoassay (ELISA).

 

Selection of suitable cell lines according to the administration route, biodistribution and expected clearance.

Cell viability assay: MTS in metabolically active cells as indicator of proliferation. Discrimination of viable and non‐viable cells using propidium iodide and trypan blue. xCELLigence system for label-free and real-time monitoring of cell -viability.

Apoptosis: Annexin V / propidium iodide, caspase 3 and caspase 8 activation by flow cytometry Reactive Oxygen Species: determination of reactive oxygen species by flow cytometry.

Quantification of colony-forming units of aerobic bacteria, yeast and mold.

Quantification of endotoxin units with different methods based on the use of Limulus Amebocyte Lysate -LAL. Preliminary screening, quantification, inhibition and enhancement.

Determination of the colloidal stability and aggregation in biological media such as saline solution, culture media, cell extracts, whole-blood or serum. 

Microscopy techniques:

Size, shape, monodispersity and composition:
- Transmission Electron Microscopy (TEM).
- Scanning Electron Microscopy (SEM).

 

Elemental Analysis:

Determination of the mass fractions of carbon (C), hydrogen (H), nitrogen (N), and sulfur (S).

 

Nuclear magnetic resonance spectroscopy (NMR):

Molecular structure determination.

 

Inductively coupled plasma (ICP):

Identification and quantification of chemicals elements.

 

Zeta potential:

Measurement of surface charge of nano/microparticles in solution.

 

Dynamic light scattering (DLS):

Hydrodynamic radius and size distribution of nanoparticles in solution.

 

Fourier transform infrared spectrophotometry (FTIR):

Characterization of the major functional groups present on the surface of the sample.

X-ray photoelectron spectroscopy:

Assessing the nature and chemical state of the surface atoms of the nanostructure to depth of 5 nm (4-20 atomic layers).

 

Fluorescence spectrophotometry:

Fluorescence analysis of samples in solution.

 

Superconducting Quantum Interference Devices (SQUID):

Determination of magnetic properties of nanostructures.

 

UV-visible spectroscopy:

Quantitative analysis of analytes.

 

Total reflection x-ray fluorescence analysis (TXRF):

Qualitative and quantitative analysis of chemical elements in solid and liquid samples.

 

Thermogravimetric analysis (TGA):

Determination of the amount of weight change of a material as a function of increasing temperature in an atmosphere of nitrogen or air.

 

Mass spectrometry (MS):

Determination of the mass of molecules.

APLICATIONS

> Biosensors design
> Surfaces functionalization
> Chips biofunctionalization
> Lateral flow test
> Magnetic separation

 

 

 

 

This kit is thought to easily modify your antibodies with oligonucleotides with length varying between 10 and 100 bases preserving their biological activity. With OligoLink kit ANTIBODY you will obtain highly-purified oligonucleotide-antibody conjugates in easy and rapid way, ready to be used in a broad range of applications such as ImmunoPCR, flow cytometry, western blotting, ELISA, etc

APLICATIONS

> Immuno-PCR
> PLA (Proximity Ligation Assay)
> PEA (Proximity Extension Assay)
> ECPA (Electrochemical Proximity Assay)

 

 

 

 

Easy-to-use magnetic nanoparticle conjugation Kit for proteins. The kit guarantees the covalent immobilization of a protein of your own choice to carboxylic groups of 200 nm magnetic nanoparticles (MNPs).

APLICATIONS

> Magnetic separation
> Immunoprecipitation
> Biosensors

 

Easy-to-use magnetic nanoparticle conjugation kit for antibodies. The kit guarantees the ORIENTED covalent immobilization of IgG antibodies of your own choice to 200 nm magnetic nanoparticles (MNPs).

APLICATIONS

> Magnetic separation
> Immunoprecipitation
> Biosensors

 

 

The all-in-one solution for an easy conjugation of your oligonucleotides (10 and 100 bases) to high quality nanoparticles. This kit guarantees the ORIENTED conjugation of your oligonucleotide to high quality gold nanoparticles via a thiol group chemically introduced to either its 5´- or 3´-end.

 

APLICATIONS

> Probes in DNA-based biosensing assays 

First kit that allows ORIENTED conjugation of antibodies and his-tagged recombinant proteins on gold nanoparticles, on an easy and quick way. You will achieve better results than the ones obtained with other conjugation methods.

ORIENTED Bioconjugation of antibodies allows maximal antigen binding and favourable protein orientation meaning no loss of their biological activity. Our method also avoids nonspecific bindings increasing kit yield and sensibility in your assay.

Aplications

> Lateral flow
> Design of biosensors
> Bioconjugation
> Microscopy probe
> Immunogold

 

Magnetic particles on the nanoscale behave as superparamagnetic leading to particles with much higher magnet susceptibilities than in traditional paramagnets. We have developed unique magnetic nanoparticles in the market, covering them with gold, providing all the properties of the gold (inertness, protection against oxidation, easy to conjugate) besides to magnetism.

 image027

Our Magnetic nanoparticles coated with gold are available in sizes ranging from 50 nm to 250 nm in diameter with different surface functionalities in a variety of solvent compositions.

CITRATE STABILIZED:

Gold shell is coated with citrate anions which can be displaced with a wide range of molecules containing a thiol (SH) group.

COOH-PEG:

Gold shell is functionalized with poly(ethylene glycol)–thiol (PEG–SH) ligands (3000 and 5000 Da) having a carboxylic acid end group, which can be further used to immobilize covalently biomolecules by formation of stable amide bonds with primary amines.

 

Aplications

> Magnetic separation
> Magnetic hyperthermia
> Biosensors
> Flow cytometry
> Drug delivery
> Lateral flow tests
> Cancer photothermal therapy
> Conductors in electronic chips

 

We offer Silver nanoparticles that can be used in applications related to their anti-microbial properties, high electrical conductivity, and optical properties. We produce the smallest PEGylated silver nanoparticles in the market

image024 image025

Our Gold nanoparticles are available in sizes ranging from 20 nm to 35 nm in diameter with COOH-PEG surface functionalization:

  • COOH-PEG: Covalent attachment of (bio)molecules containing primary amines, -NH2.

You can use them for:

  • Biosensors and Immunoassays
  • Anti-bacterial
  • Conductors in electronic chips
  • Metal-enhanced fluorescence (MEF)
  • SERS

We commercialize high quality Gold nanostars, formed by multiple branches with sharp tips with highly-competitive characteristics guarantying to meet specifications.

TEM Microscopy

image020 image021

Our Gold nanostars are available in size 40 nm but can be produced in other sizes upon request:

  • PVP STABILIZED: Polyvinylpyrollidone (PVP) is a polymer that binds strongly to the gold nanoparticle surface providing greater stability than citrate or tannic acid.
  • SILICA COATED: silica coating confers thermodynamic and colloidal stability to gold nanostars. 

You can use them for:

  • Surface Enhanced Raman Scattering (SERS)
  • Biosensors

We offer you one of the smallest possible gold probes (0.8nm – 3nm), ideal for ultra-high-resolution EM applications. They have been extensive characterized (TEM, UV-Visible spectroscopy and DLS data), demonstrating a narrow size distribution.

TEM Microscopy

 image012 image010

Intrinsic fluorescence

image016image017

 

Our Gold nanoclusters are available in sizes ranging from 0.8 nm to 3 nm with different surface functionalities in a variety of solvent compositions.

  • CARBOXILATED :can be activated by EDC and NHS to form an intermediate that can subsequently react with primary amine groups on a specific protein or any other ligand to be conjugated
  • THIOPRONINE :
  • AMINE: prepared for binding to the negative charges of DNA backbone

Customer conjugation is available upon request.

You can use them for:

  • Probes for molecular biology or electron microscopy
  • Antibody labelling
  • Bioelectrochemistry
  • Biosensors

It is commonly known that the properties of Gold nanoparticles depend strongly upon their size and shape. Our Gold nanoparticles are exhaustive characterized (TEM, DLS, UV-Vis, Z-potential measurements), possess high monodispersity (CV<15%) and great stability.

Our Gold nanoparticles are available in sizes ranging from 3 nm to 100 nm in diameter with different surface functionalities in a variety of solvent compositions.

CITRATE STABILIZED:

Attachment of (bio)molecules containing thiols groups –SH.

COOH-PEG:

Covalent attachment of (bio)molecules containing primary amines, -NH2.

LIPOIC ACID:

Covalent attachment of (bio)molecules containing primary amines, -NH2.

SILICA COATED:

Silica coating confers thermodynamic and colloidal stability to gold nanoparticles. It also increases the surface area for conjugation of biomolecules, or, as a porous material, can be loaded with drugs, dye molecules, or other imaging agents either via physical adsorption or covalent attachment. Lastly, the silica-coating on gold nanoparticles can enhance imaging contrast by more than 3 fold.

Aplications

> Diagnostics-Immunoassays
> Biosensors
> Probes for molecular biology or electron microscopy
> Drug delivery
> Catalyst in chemical reactions
> Conductors in electronic chips

CAT# PRODUCT NAME SIZE PRICE/ €
51011915R Citrate stabilized 250nm

temporarily discontinued

245,00
51011915S Citrate stabilized 250nm

temporarily discontinued

326,00
51011915Y Citrate stabilized 250nm

temporarily discontinued

544,00
51011915W Citrate stabilized 250nm

temporarily discontinued

470,00
51011315S Citrate stabilized 25 nm

1 ml

144,99
51011315W Citrate stabilized 25 nm 5ml

5 ml

294,99
51011315Y Citrate stabilized 25 nm 10 ml

10 ml

820,00
51011515S Citrate stabilized 40 nm

1 ml

197,70
51011515W Citrate stabilized 40 nm 5ml

5 ml

392,46
51011515Y Citrate stabilized 40 nm 10ml

10 ml

616,31
51011415S Carboxylated 25 nm

1 ml

301,99
51011415W Carboxylated 25 nm 5ml

5 ml

913,86
51011415Y Carboxylated 25 nm 10ml

10 ml

1.524,38
51011615S Carboxylated 40 nm

1 ml

228,79
51011615W Carboxylated 40 nm 5ml

5 ml

547,91
51011615Y Carboxylated 40 nm 10ml

10 ml

927,23
51000317V Citrate stabilized 3nm -1 OD

25 ml

60,00
510003160 Citrate stabilized 3nm - 1 OD

100 ml

125,00
51000316C Citrate stabilized 3nm - 1 OD

250 ml

240,00
51000316F Citrate stabilized 3nm - 1 OD

500 ml

400,00
51000417V Citrate stabilized 12nm - 1 OD

25 ml

60,00
510004160 Citrate stabilized 12nm - 1 OD

100 ml

125,00
51000416C Citrate stabilized 12nm - 1 OD

250 ml

240,00
51000416F Citrate stabilized 12nm - 1 OD

500 ml

400,00
51002415S Carboxylated 0.8 nm 1ml

1 ml (8 nmol)

240,00
51002415W Carboxylated 0.8 nm 5ml

1 ml (40 nmol)

310,00
51002417V Carboxylated 0.8 nm 25ml

25 ml (200 nmol)

659,99
51002515S Carboxylated 1.5 nm 1ml

1 ml (1,6 nmol)

265,00
51002515W Carboxylated 1.5 nm 5ml

5 ml (8 nmol)

384,99
51002517V Carboxylated 1.5 nm 25ml

25 ml (40 nmol)

969,99
51002615S Carboxylated 2 nm 1ml

1 ml (0,8 nmol)

285,00
51002615W Carboxylated 2 nm 5ml

5 ml (4 nmol)

440,00
51002617V Carboxylated 2 nm 25ml

25 ml (20 nmol)

1.200,00
51008715S Thiopronine 3 nm 1ml

1 ml

290,00
51008715W Thiopronine 3 nm 5ml

5 ml

465,00
51008717V Thiopronine 3 nm 25ml

25 ml

1.320,00
51009815S Amine 1.5 nm 1ml

1 ml

295,00
51009815W Amine 1.5 nm 5ml

5 ml

494,99
51009817V Amine 1.5 nm 25ml

25 ml

1.374,99
Custom PVP stabilized Gold nanostars

10 mg

Custom Silica coated Gold nanostars

10 mg

51010615Y COOH-PEG 5000Da 20nm

10 ml

114,59
510106161 COOH-PEG 5000Da 20nm 20ml

20 ml

201,21
51010815Y COOH-PEG 5000Da 25nm

10 ml

114,59
510108161 COOH-PEG 5000Da 25nm 20ml

20 ml

201,21
51011015Y COOH-PEG 5000Da 30nm

10 ml

114,59
510110161 COOH-PEG 5000Da 30nm 20ml

20 ml

201,21
51011215Y COOH-PEG 5000Da 35nm

10 ml

114,59
510112161 COOH-PEG 5000Da 35nm 20ml

20 ml

201,21
51010515Y COOH-PEG 3000Da 20nm

10 ml

110,50
510105161 COOH-PEG 3000Da 20nm 20ml

20 ml

193,00
51010715Y COOH-PEG 3000Da 25nm

10 ml

110,50
510107161 COOH-PEG 3000Da 25nm 20ml

20 ml

193,00
51010915Y COOH-PEG 3000Da 30nm

10 ml

110,50
510109161 COOH-PEG 3000Da 30nm 20ml

20 ml

193,00
51011115Y COOH-PEG 3000Da 35nm

10 ml

110,50
510111161 COOH-PEG 3000Da 35nm

20 ml

193,00
51008315S Citrate stabilized 50nm

1 mL in stock

80,00
51008315W Citrate stabilized 50nm

5 mL  in stock

210,00
51008317V Citrate stabilized 50nm

25 mL in stock

740,00
51008415S COOH-PEG 3000Da 50nm

1 mL (available on demand)

145,00
51008415W COOH-PEG 3000Da 50nm

5 mL (available on demand)

295,00
51008417V COOH-PEG 3000Da 50nm

25 mL (available on demand)

820,00
51008515S COOH-PEG 5000Da 50nm

1 mL (available on demand)

145,00
51008515W COOH-PEG 5000Da 50nm

5 mL (available on demand)

295,00
51008517V COOH-PEG 5000Da 50nm

25 mL (available on demand)

820,00
51000517V Citrate stabilized 12nm - 3 OD

25 ml

89,99
51000517V Citrate stabilized 12nm - 3 OD

25 ml

89,99
510005160 Citrate stabilized 12nm - 3 OD

100 ml

279,99
51000516C Citrate stabilized 12nm - 3 OD

250 ml

614,00
51000617V Citrate stabilized 20nm - 1 OD

25 ml

60,00
510006160 Citrate stabilized 20nm - 1 OD

100 ml

125,00
51000616C Citrate stabilized 20nm - 1 OD

250 ml

240,00
51000616F Citrate stabilized 20nm - 1 OD

500 ml

400,00
51000717V Citrate stabilized 20nm - 3 OD

25 ml

89,99
510007160 Citrate stabilized 20nm - 3 OD

100 ml

279,99
51000716C Citrate stabilized 20nm - 3 OD

250 ml

614,00
51000817V Citrate stabilized 25nm - 1 OD

25 ml

60,00
510008160 Citrate stabilized 25nm - 1 OD

100 ml

125,00
51000816C Citrate stabilized 25nm - 1 OD

250 ml

240,00
51000816F Citrate stabilized 25nm - 1 OD

500 ml

400,00
51000917V Citrate stabilized 25nm - 3 OD

25 ml

89,99
510009160 Citrate stabilized 25nm - 3 OD

100 ml

279,99
51000916C Citrate stabilized 25nm - 3 OD

250 ml

614,00
51001017V Citrate stabilized 30nm - 1 OD

25 ml

60,00
510010160 Citrate stabilized 30nm - 1 OD

100 ml

125,00
51001016C Citrate stabilized 30nm - 1 OD

250 ml

240,00
51001016F Citrate stabilized 30nm - 1 OD

500 ml

400,00
51001117V Citrate stabilized 30nm - 3 OD

25 ml

89,99
5100011160 Citrate stabilized 30nm - 3 OD

100 ml

279,99
51001116C Citrate stabilized 30nm - 3 OD

250 ml

614,00
51001217V Citrate stabilized 35nm - 1 OD

25 ml

60,00
510012160 Citrate stabilized 35nm - 1 OD

100 ml

125,00
51001216C Citrate stabilized 35nm - 1 OD

250 ml

240,00
51001216F Citrate stabilized 35nm - 1 OD

500 ml

400,00
51001317V Citrate stabilized 35nm - 3 OD

25 ml

89,99
510013160 Citrate stabilized 35nm - 3 OD

100 ml

279,99
51001316C Citrate stabilized 35nm - 3 OD

250 ml

614,00
51001417V Citrate stabilized 40nm - 1 OD

25 ml

60,00
510014160 Citrate stabilized 40nm - 1 OD

100 ml

125,00
51001416C Citrate stabilized 40nm - 1 OD

250 ml

240,00
51001416F Citrate stabilized 40nm - 1 OD

500 ml

400,00
51001517V Citrate stabilized 40nm - 3 OD

25 ml

89,99
510015160 Citrate stabilized 40nm - 3 OD

100 ml

279,99
51001516C Citrate stabilized 40nm - 3 OD

250 ml

614,00
51003117V Citrate stabilized 50nm - 1 OD

25 ml

60,00
510031160 Citrate stabilized 50nm - 1 OD

100 ml

125,00
51003116C Citrate stabilized 50nm - 1 OD

250 ml

240,00
51003116F Citrate stabilized 50nm - 1 OD

500 ml

400,00
51003217V Citrate stabilized 50nm - 3 OD

25 ml

89,99
510032160 Citrate stabilized 50nm - 3 OD

100 ml

279,99
51003216C Citrate stabilized 50nm - 3 OD

250 ml

614,00
51003317V Citrate stabilized 60nm - 1 OD

25 ml

60,00
510033160 Citrate stabilized 60nm - 1 OD

100 ml

125,00
51003316C Citrate stabilized 60nm - 1 OD

250 ml

240,00
51003316F Citrate stabilized 60nm - 1 OD

500 ml

400,00
51003417V Citrate stabilized 60nm - 3 OD

25 ml

89,99
510034160 Citrate stabilized 60nm - 3 OD

100 ml

279,99
51003416C Citrate stabilized 60nm - 3 OD

250 ml

614,00
51003517V Citrate stabilized 70nm - 1 OD

25 ml

60,00
510035160 Citrate stabilized 70nm - 1 OD

100 ml

125,00
51003516C Citrate stabilized 70nm - 1 OD

250 ml

240,00
51003516F Citrate stabilized 70nm - 1 OD

500 ml

400,00
51003617V Citrate stabilized 70nm - 3 OD

25 ml

89,99
510036160 Citrate stabilized 70nm - 3 OD

100 ml

279,99
51003616C Citrate stabilized 70nm - 3 OD

250 ml

614,00
51003717V Citrate stabilized 80nm - 1 OD

25 ml

60,00
510037160 Citrate stabilized 80nm - 1 OD

100 ml

125,00
51003716C Citrate stabilized 80nm - 1 OD

250 ml

240,00
51003716F Citrate stabilized 80nm - 1 OD

500 ml

400,00
51003817V Citrate stabilized 80nm - 3 OD

25 ml

89,99
510038160 Citrate stabilized 80nm - 3 OD

100 ml

279,99
51003816C Citrate stabilized 80nm - 3 OD

250 ml

614,00
51003917V Citrate stabilized 90nm - 1 OD

25 ml

60,00
510039160 Citrate stabilized 90nm - 1 OD

100 ml

125,00
51003916C Citrate stabilized 90nm - 1 OD

250 ml

240,00
51003916F Citrate stabilized 90nm - 1 OD

500 ml

400,00
51004017V Citrate stabilized 90nm - 3 OD

25 ml

89,99
510040160 Citrate stabilized 90nm - 3 OD

100 ml

279,99
51004016C Citrate stabilized 90nm - 3 OD

250 ml

614,00
51004117V Citrate stabilized 100nm - 1 OD

25 ml

60,00
510041160 Citrate stabilized 100nm - 1 OD

100 ml

125,00
51004116C Citrate stabilized 100nm - 1 OD

250 ml

240,00
51004116F Citrate stabilized 100nm - 1 OD

500 ml

400,00
51004217V Citrate stabilized 100nm - 3 OD

25 ml

89,99
510042160 Citrate stabilized 100nm - 3 OD

100 ml

279,99
51004216C Citrate stabilized 100nm - 3 OD

250 ml

614,00
51002717V Lipoic acid 3nm - 1 OD

25 ml

77,99
510027160 Lipoic acid 3nm - 1 OD

100 ml

142,99
51002716C Lipoic acid 3nm - 1 OD

250 ml

271,99
51002716F Lipoic acid 3nm - 1 OD

500 ml

491,99
51002817V Lipoic acid 3nm - 3 OD

25 ml

121,00
510028160 Lipoic acid 3nm - 3 OD

100 ml

313,99
51002816C Lipoic acid 3nm - 3 OD

250 ml

1.344,99
Custom Silica coated Gold nanoparticles
510017155 COOH-PEG 3000Da 12nm

1 ml

350,00
51001715W COOH-PEG 3000Da 12nm

5 ml

1.290,00
51001915S COOH-PEG 5000Da 12 nm

1 ml

350,00
51001915W COOH-PEG 5000Da 12 nm

5 ml

1.290,00
51002115S COOH-PEG 3000Da 30 nm

1 ml

350,00
51002115W COOH-PEG 3000Da 30 nm

5 ml

1.290,00
51002315S COOH-PEG 5000Da 30 nm

1 ml

350,00
51002315W COOH-PEG 5000Da 30 nm

5 ml

1.290,00